Confocal Microscopy Illinois State University Advanced Bioimaging

Confocal Microscope Leica Sp8 Set Up With Computer. Leica SP8 upright confocal Gurdon Institute Note: using focus knob on microscope or joystick will not adjust galvostage position for z-stack Press the XYZ icon on the Microscope LCD display to change to Stage mode

Note: using focus knob on microscope or joystick will not adjust galvostage position for z-stack Press the Z Fine button on the joystick base, and fine tune the focus of your specimen

LEICA SP8 Confocal Microscope (Advanced) Humanitas Research

It also gives guidance on acquiring images including setting up sequential scans and adjusting parameters like format, speed and pinhole size. Press the XYZ icon on the Microscope LCD display to change to Stage mode However, for example, some sub-cellular structures of interest may be beyond Abbe's diffraction limit and therefore beyond the microscope's capability to resolve.

Confocal Microscopy Illinois State University Advanced Bioimaging. Pinhole (Confocal Aperture) Ideally, the confocal aperture should be set to the size of the structure(s) you are trying to resolve The TCS SP8 MP is equipped with two automated stages, a scan head, an electro-optical modulator, a monitor and computer, and a tunable near-IR laser.

Leica SP8 up (WLL,FALCON) Imaging and Optics Facility. The document provides instructions for start up and operation of a Leica SP8 TCS confocal microscope Turn on switches for the UV light source (5) and the power strip (6) supplying the camera